| 发明名称 |
HEPARINASE GENE FROM FLAVOBACTERIUM HEPARINUM |
| 摘要 |
2122004 9308289 PCTABS00021 The cloning of the heparinase gene from Flavobacterium Heparinum) using the polymerase chain reaction is described. The Open Reading Frame (ORF) corresponded to 1152 base pairs encoding a precursor protein of MW 43,800 daltons. The amino acid sequence reveals a 20-residue leader peptide. The gene was expressed in two expression systems in E. Coli.
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| 申请公布号 |
CA2122004(A1) |
申请公布日期 |
1993.04.29 |
| 申请号 |
CA19922122004 |
申请日期 |
1992.10.22 |
| 申请人 |
MASSACHUSETTS INST TECHNOLOGY |
发明人 |
SASISEKHARAN RAMNATH;MOREMEN KELLEY;COONEY CHARLES L;ZIMMERMAN JOSEPH L;LANGER ROBERT S |
| 分类号 |
C12N15/09;C12N1/21;C12N9/88;C12N15/00;C12N15/60;C12R1/01;C12R1/20;(IPC1-7):C12N15/60 |
主分类号 |
C12N15/09 |
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