| 摘要 |
PURPOSE:To reduce the quantity of a specimen, by a method wherein the antibodies corresponding to a plural kinds of antigens to be measured are respectively immobilized on different membrane like carriers and, after the carriers are put one upon another, a specimen to be examined is moved in a plurality of the carriers to generate antigen-antibody reaction. CONSTITUTION:A glass ring 1 subjected to silane coupling treatment is put on an acrylic resin plate and a polymer solution is rapidly dripped from above and, further, another acrylic resin plate is put on the ring 1 so as to hold said ring 1 between both resin plates. After the polymer solution is gelled, the acrylic resin plates are detached to obtain an immobilized antibody film 2 having a polyacrylamide gel bonded thereto wherein an antibody is immobilized on the hole part of the ring 1. Next, different kinds of antibodies are used to similarly prepare various immobilized antibody films 3, 4. Subsequently, the antibody films 2-4 to various kinds of antigens are put one upon another and the labeled antigens corresponding to the respective ones are moved by electrophoresis to be reacted with the antibodies and the concn. of each of the labeled antigens is measured at every electrophoretic carrier. By this method, the quantity of a liquid to be examined can be reduced. |
