发明名称 Process for the manufacture of a biosensor employing an enzyme as active molecule, and biosensor obtained by this process
摘要 To prepare a biosensor comprising an insulating support 1 provided with a conductive film 4 acting as electrodes, on which a layer 5 is arranged comprising active molecules A, for example glucose peroxidase, a monomolecular layer of amphiphilic molecules B coupled with the molecules A is deposited on the support provided with the conductive film 4. The Langmuir Blodgett method is employed for this purpose with a bath containing the molecules A and a compression of the monomolecular layer formed on the bath is carried out, with a view to its deposition on the support, with a high surface tension P, for example of 45 mN/m. This makes it possible to obtain a compact organisation of the molecules A and to make them more accessible to the compound to be detected. The performance of the biosensor can be improved further by carrying out a surface treatment of the film 4 before the deposition of the layer 5. <IMAGE>
申请公布号 FR2682765(A1) 申请公布日期 1993.04.23
申请号 FR19910012764 申请日期 1991.10.16
申请人 COMMISSARIAT A ENERGIE ATOMIQUE;BIOTICA INTERNATIONAL;CENTRE NAL RECHERC SCIENTIFIQUE 发明人 BARRAUD ANDRE;VALLETON JEAN-MARC;PERRIN YVES;FIOL CATHERINE
分类号 C12Q1/00 主分类号 C12Q1/00
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