发明名称 |
Purified immunoglobulin. |
摘要 |
Following are described. (A) purified prepn. of an anti-CDw52 antibody which exhibits on size exclusion chromatography a single peak under non-reducing conditions and 2 major peaks under reducing conditions; esp. the prepn. exhibits on conventional SDS polyacrylamide gel electrophoresis, one main band using a non-reduced sample and two main bands using a reduced sample; (B) purified prepn. of an anti-CDw52 antibody having a specific activity greater than 0.8 KU/mg; (C) purified prepn. of an anti-CDw52 antibody, free from host cell contaminants and/or aggregates; (D) purifying an anti-CDw52 antibody comprising (a) applying an aqs. soln. of the antibody to a Protein A column so as to absorb the antibody and eluting the antibody with an acid soln., (b) applying the acidic eluate to an ion-exchange column of charged particles to absorb the antibody and then eluting the antibody with an aqs. soln. of counter-charged ions and (c) applying the aqs. eluate to a size exclusion column of porous particles to retain non-antibody mols. in the porous particles and to obtain the desired antibody in selected fractions produced from the column. Pref. the Protein A column is Protein A Sepharose and the antibody is eluted with citric acid. |
申请公布号 |
ZA9206191(B) |
申请公布日期 |
1993.04.16 |
申请号 |
ZA19920006191 |
申请日期 |
1991.10.16 |
申请人 |
THE WELLCOME FOUNDATION LIMITED |
发明人 |
PAUL IAN NICHOLAS RAMAGE;GEOFFREY ALLEN |
分类号 |
A61K39/395;A61K;A61K38/00;C07K;C07K1/16;C07K1/18;C07K1/20;C07K1/34;C07K1/36;C07K14/00;C07K16/00;C07K16/06;C07K16/24;C07K16/28;C07K16/42;C12N15/09;C12N15/13;C12P21/08;C12R1/91 |
主分类号 |
A61K39/395 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|