| 摘要 |
<p>A substantially purified O-CSF protein capable of stimulating growth of TRAP-positive osteoclast progenitors in bone marrow cell cultures, and an isolated cDNA coding for O-CSF. Osteoclast progenitors grown in the presence of the O-CSF protein exhibit bone resorption activity. The O-CSF protein has an apparent molecular weight in the 20-25kD range, with a peak of biological activity at approximately 20kD, as determined by SDS-PAGE electrophoresis and biological assay. The O-CSF protein may be purified as disclosed herein from the culture medium of CESJ-3 cells (ATCC No. CRL 10886). The O-CSF protein substantially purified in this manner has an osteoclast progenitor stimulating activity of at least 660,000 units per milligram of protein.</p> |
