发明名称 |
Sol capture immunoassay kit and procedure |
摘要 |
An immunoassay procedure for detection of analytes in urine wherein an immunological reaction is conducted in an aqueous phase containing the urine and a filterable immunocomposite containing an inherently colored gold sol particle is formed if the assay is positive. The colored, gold sol particle containing immunocomposite is collected for direct visual observation on a filter element. False positive readings are minimized by (1) removing contaminants from the urine to prevent clogging of the filter element and non-specific binding of unreacted reactants thereto by contacting the urine with a porous contact element having a detergent material dispersed on its external and interstitial surfaces; (2) preventing non-specific binding of unreacted immunoreactive substances to the filter element by blocking the latter with polyvinyl pyrrolidone; and/or (3) including a green dye material in the aqueous reaction phase to improve the optical distinction after washing the collection filter between negative and positive results on the filter.
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申请公布号 |
US5202267(A) |
申请公布日期 |
1993.04.13 |
申请号 |
US19880177114 |
申请日期 |
1988.04.04 |
申请人 |
HYGEIA SCIENCES, INC. |
发明人 |
DITLOW, CHARLES C.;CANNON, L. EDWARD;COLE, FRANCIS X.;DAVIS, GENE A.;SIGILLO, ERIC C.;DANTI, ALICIA G. |
分类号 |
G01N33/53;G01N33/543;G01N33/58 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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