发明名称 |
Expression cloning method. |
摘要 |
<p>Disclosed is a process for cloning a nucleotide sequence that encodes a protein. The process includes the steps of: preparing a recombinant molecule containing the nucleotide sequence and a vector that includes an expression control element operatively attached at linkage sites to the nucleotide sequence; introducing the recombinant molecule into host cells derived from a multi-cellular organism; identifying, among the host cells into which the recombinant molecule is introduced, cells expressing the protein by a means that specifically detects the protein; attaching the cells expressing the protein to a solid surface; and recovering the recombinant molecule from the attached cells. The same process can also be used to test the specificity of an antibody in its binding to an antigen, when a DNA sequence encoding the antigen is available. <IMAGE></p> |
申请公布号 |
EP0534619(A2) |
申请公布日期 |
1993.03.31 |
申请号 |
EP19920307795 |
申请日期 |
1992.08.26 |
申请人 |
RHODE ISLAND HOSPITAL |
发明人 |
SANG, JUNSHENG;THOMPSON, NANCY L.;HIXSON, DOUGLAS C. |
分类号 |
C12N11/02;C12N9/12;C12N15/09;C12N15/64;C12N15/85;G01N33/53 |
主分类号 |
C12N11/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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