FUER EIN LYTISCH WIRKSAMES, CHIMAERES PROTEIN KODIERENDE REKOMBINANTE DNA UND IHRE VERWENDUNG.

摘要

New recombinant DNA (I) comprises (a) the sequence encoding the N-terminal, membrane-penetrating domain of the E-protein of phage phix 174; (b) the sequence encoding the C-terminal, membrane-penetrating domain of the L-protein of phage MS2; these two sequences being joined by (c) a sequence encoding a hydrophilic, flexible amino acie sequence. Also new is plasmid pRM17 (D5M 4092P) contg. a recombinant sequence encoding for the protein of formula: MVRWTLWDTLAFLLLLSLLLPSLLIMF IPSTFKRPVSSWKALNLR KTLLMASSVGDPLGPFKHE DYPCRRQQRSSTLYULIFLAIFLSKFT NQLLLSLLEAVIRTVTTL QQLLT. The underlined pentapeptide is sequence (c).