Cloned human centromere autoantigen

摘要

The invention relates to DNA molecules coding for human CENP-B polypeptides. The invention provides DNA molecules comprising an epitopically functional part of the cDNA sequence of human CENP-B polypeptide. The invention also provides cloning vehicles capable of replication and expression comprising cDNA molecules coding for human CENP-B polypeptide. The invention further provides for hosts transformed with a vehicle having a cDNA molecule coding for human CENP-B polypeptide. In another embodiment, the invention provides for the detection of autoantibodies to human CENP-B using the human CENP-B polypeptide encoded for by the cDNA molecules of the invention. Also, the invention provides monoclonal antibodies, and hybridomas which produce them, which bind an epitope on human CENP-B which corresponds to an epitope to which autoantibodies to human CENP-B bind.