| 摘要 |
PURPOSE:To obtain the subject compound in an extremely good yield in a short time by treating the racemate of a carboxylate ester with a transformed microorganism containing a DNA fragment coding a specific esterase having excellent thermal stability. CONSTITUTION:A host microorganism is transformed with a recombinant ligated with a DNA fragment comprising a base sequence of formula I originated from Pseudomonas putida MR-2068 (FERN P9677) or its part to obtain a transformant. The cultured solution of the transformed microorganism or its cells (treated product) is acted on the racemate of a carboxylate ester of formula II (R1 is alkyl, aryl, etc.; R2, R3 are alkyl; (n) is 1, 2) preferably at a pH of 5-8 at a reaction temperature of >=40 deg.C to obtain the objective compound of formula III. |
