USE OF SELF-PROMOTER FOR EXPRESSING GENE PRODUCT IN BORDETELLA BACTERIA

摘要

Autologous promoters are used to effect expression of gene products in pertissis strains. Hybrid pertussis genes are constructed comprising a structural pertussis gene fused at an ATG codon to a native but autologous pertussis promoter. B. pertussis, transformed by the hybrid gene by insertion into the chromosome of the organism by homologous recombination at specific loci, effects expresion of the antigenic protein for which the structural gene codes at a production rate different from that achieved for the homologous gene. Specific strains and plasmids are described. The technique is applicable to a wide variety of gene products and organisms.