RECOMBINANT PLASMID HAVING NITRILE DECOMPOSING ENZYMIC GENE, TRANSFORMED MICROORGANISM AND PRODUCTION OF AMIDE AND ACID BY THE SAME TRANSFORMED MICROORGANISM

摘要

PURPOSE:To obtain the subject plasmid capable of remarkably enhancing catalyst ability and efficiently converting a nitrile into an amide and an acid by ligating a nitrile decomposing enzymic gene derived from a bacterium of the genus Rhodococcus to a specific complex plasmid vector. CONSTITUTION:One or plural nitrile decomposing enzymic gene DNAs derived from a bacterium of the genus Rhodococcus are ligated to a complex plasmid vector containing a DNA region, replicable and proliferative in a strain cell belonging to the genus Rhodococcus, a plasmid DNA region, replicable and proliferative in an Escherichia coli cell and a DNA region containing a medicine- resistant gene to prepare a recombinant plasmid, which is then transduced into a microorganism belonging to the genus Rhodococcus. Transformation is carried out and the resultant transformed microorganism is cultured in a culture medium containing a nitrile (e.g. acrylonitrile). Thereby, conversion from the nitrile into an amide and further an acid is performed with high efficiency.