Immunoassays for thyroid hormones using thyroglobulin

摘要

Immunoassays for measuring thyroid hormones such as thyroxine(T4) and triiodothyronine(T3) are carried out by incubating a serum sample with either (a) unlabeled thyroglobulin and a labeled antibody to a thyroid hormone or to thyroxine binding globulin(TBG), or (B) labeled thyrogloublin and an unlabeled antibody to a thyroid hormone. The thyroglobulin may be immobilized on an insoluble carrier and the immobilized thyroglobulin is preferably modified by succinylation with succinic anhydride. Whole thyroglobulin can be used or the thyroglobulin can be fragmented into smaller peptides prior to use provided the peptides contain at least one T3 or T4 residue per peptide. Preferably, the antibody is a monocional antibody and labeling is with an acridinium ester.