| 摘要 |
PURPOSE:To obtain the subject polymer useful for bio-decomposable medical material, etc., in high yield by culturing a microbial strain belonging to genus Alcaligenes and capable of producing a polyester containing 3-hydroxybutyrate in a medium containing a long-chain fatty acid and collecting the product accumulated in the microbial cell. CONSTITUTION:A microbial strain belonging to genus Alcaligenes and capable of producing a polyester containing 3-hydroxybutyrate [e.g. Alcaligenes lipolytica AK201 (FERM P-12198)] is cultured in a liquid medium containing at least one kind of compound selected from long-chain fatty acid (e.g. undecanoic acid) and its derivative at 30 deg.C for 48hr under shaking at a rate of 130 strokes per minute. The culture liquid is subjected to centrifugal separation, washed with water and the collected microbial cells are freeze-dried. The freeze-dried cells are treated with hot chloroform to extract the polyester accumulated in the cell, the extracted liquid is concentrated, added with hexane and the precipitate is filtered and dried to obtain the objective bio-polyester composed of a copolymer of 3-hydroxybutyrate and 3-hydroxyvaleric acid, etc. |
