Purificn. of ganglioside mixt. - using solvent extn. and PPTN processes, to obtain mixt. for use in repair of peripheral and central nervous systems

摘要

Prepn. of a mixt. of gangliosides comprises (a) subjecting ganglioside-contg. tissue to lipid elimination with acetone to produce an acetone ppte., (b) suspending the acetone ppte. in a first solvent mixt. (e.g. CH2Cl2, MeOH an NaOH) capable of partitioning hydrophobic substances from hydrophilic substances, (c) filtering the partitioning mixt. to obtain a first liquid phase, (d) subjecting the first liquid phase to pptn. (e.g. by adding CaCl2 and acetone) to obtain a first raw material, (e) solubilising the first raw material (using e.g. a mixt. of water, CHCl3 and MeOH) and subjecting the solubilised first raw material to heating at a pH of about 12, (f) subjecting the heated solubilised first raw material to a second partitioning in a second solvent mixt. (e.g. water, CHCl3 and n-butanol) capable of partitioning hydrophobic substances from hydrophilic substances, (g) sepg. the second partitioning mixt. to remove an organic phase and retain an aqs. phase, (h) subjecting the aqs. phase to pptn. (e.g. by adding acetone and NaCl) to produce a second raw material, (j) solubilising the third raw material in a base, e.g. 1N NaOH, (k) neutralising the solubilised third raw material and (l) subjecting the neutralised solubilised third raw material to dialysis through a membrane with a mol. wt. cut off of about 10 kD to produce a ganglioside mixt.. Also claimed is a ganglioside mixt. prepd. by the method.