IMPROVEMENTS IN OLIGONUCLEOTIDE PRIMERS AND PROBES

摘要

This invention relates to oligonucleotides comprising one or more degenerate base analogues of the structure shown in Figure 2 in which R = H or NH2 and R' = H or CH3, for use as primers e.g. in PCR and as hybridisation probes. Also disclosed are methods of performing DNA sequencing reactions or the polymerase chain reaction and hybridisation reactions, involving use of oligonucleotides containing one or more degenerate base analogues of the present invention.