| 摘要 |
<p>A multiple base addition sequencing scheme (BASS) for the rapid sequencing of oligonucleotides (DNA and RNA) involving the steps of attaching a plurality of DNA or RNA strands to be sequenced to a coated support, adding a modified nucleotide, detecting the modified nucleotide, removing the blocking group and repeating the steps until the RNA or DNA is sequenced. Methods for improving and monitoring coupling efficiency are included. Two separate methods of automation using BASS are described. The first includes buffer reservoirs, two controllable valves, a detector and a plurality of reaction columns. The second includes a solid support, a plurality of buffer reservoirs, a detector means, a computer means and a movement means. Also described is a device for simultaneously sequencing a plurality of RNA or DNA sequences.</p> |
