A DNA sequence of high biotin operon-expression system usable for breeding a bacterium having excellent biotin productivity is provided. A DNA sequence of biotin operon characterized in the fact that at least one base pair of either a nucleotide sequence of the regulatory region of the biotin operon of Escherichia coli or a nucleotide sequence in the vicinity of the bioB initiating codon is mutated in comparison with that of the one in its wild type strain is provided. Escherichia coli transformed with a recombinant plasmid carrying such a DNA sequence has high biotin-productivity.
申请公布号
EP0532426(A2)
申请公布日期
1993.03.17
申请号
EP19920402495
申请日期
1992.09.11
申请人
SHISEIDO COMPANY LIMITED;TAKEDA CHEMICAL INDUSTRIES, LTD.