Method for preparing chitosan.

摘要

According to the method for preparing chitosan of the present invention, chitosan is prepared by inoculating a filamentous fungi belonging to the family Mucoraceae into a culture medium for pre-cultivation containing yeast extract, malt extract, peptone, glucose and magnesium sulfate to perform pre-cultivation and then inoculating the pre-culture into a culture medium for main-cultivation having a composition essentially identical to that of the medium for pre-cultivation to perform the main-cultivation. Particularly preferred filamentous fungi belonging to the family Mucoraceae is Absidia coerulea. According to the method for preparing chitosan through the cultivation of microorganisms, chitosan exhibiting uniform quality can efficiently be produced and the method does not adversely affect the circumferential environment.