发明名称 Process for the incorporation of DNA molecules into microorganisms with methyl-specific restriction systems
摘要 There is disclosed a process for the incorporation of DNA into a microorganism that, because of natural restrictions in the organism, normally would prevent or limit the incorporation of the DNA. The process involves the selection of an intermediate host organism that has no restrictions to the incorporation of DNA. The DNA is then transferred into the intermediate host which is readily accomplished because there are no restrictions. The DNA is then isolated from the intermediate host and transferred into the target organism. Using this process the natural restrictions of the host organism are circumvented and DNA is transferred from a source organism to a target organism that normally would not be possible. In this manner desirable characteristics from the source organism can be imparted into the target organism.
申请公布号 US5194387(A) 申请公布日期 1993.03.16
申请号 US19910792998 申请日期 1991.11.15
申请人 MERCK & CO., INC. 发明人 MACNEIL, DOUGLAS J.
分类号 C07K14/36;C12N15/64;C12N15/76 主分类号 C07K14/36
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