Affinity carrier for fractionation of bio-polymers - comprises polyamide grafted with vinyl monomer carrying coupling side gps. and monomer with non-coupling gps.

摘要

In an affinity carrier based on polyamides onto which polymers have been grafted, the grafted polymers are (a) 70-98% from vinyl monomers with functional side-gps. capable of coupling of formula CH2=CR1-COX and (b) 30-2% of co-monomers with non-coupling gps. R1 is H or Me, X is OH- 0(CH2)2-6-OH, -NHOH, gp. (i). Pref. comonomers are N-vinylpyrrolidone, (meth)acrylamide, maleic anhydride and/or allyl urea, esp. 0.05-5% of vinyl monomer with at least 2 reactive vinyl functions or may be acrolein. The graft copolymer may contain an amino gp. formed by reaction of a graft copolymer contg. an oxirane gp. with a diamine. USE/ADVANTAGE - The affinity carrier is used in fractionation of biopolymers (claimed) by affinity and immuno-affinity chromatography and in immobilisation of bio-molecules. Ligands in the form of peptides, proteins, enzymes, antibodies, blood factors, nucleotides, nucleic acids, glycoproteins and oligosaccharides can be covalently bonded onto the matrix. The carrier is mechanically stable. .