Method of measuring NADH and NADPH.

摘要

There is disclosed a method of measuring NADH or NADPH in a body fluid such as serum for quantitatively measuring a biochemical substance or activity of enzyme therein. According to the method, steps of generating hydrogen peroxide by an action of NADH or NADPH oxidase and measuring the hydrogen peroxide by a chromogenic reaction are concurrently carried out. As the color developing reagent of oxidative coupling reaction, peroxidase, a substance selected from aniline and its derivative, and a substance selected from 3-methyl-2-benzothiazolinone hydrazone and its derivative are employed in combination. If necessary, an enzyme of superoxide dismutase is coexisted in reaction system.