发明名称 |
ISOLATION OF BIOACTIVE, MONOMERIC GROWTH HORMONE |
摘要 |
<p>Monomeric, biologically active growth hormone is isolated from microbially-produced insoluble inclusion bodies by solubilizing and denaturing the growth hormone by extraction of the inclusion bodies into a guanidine salt solution such as guanidine hydrochloride and subsequently renaturing at least a portion of the growth hormone in the solution by replacing the guanidine salt solution with a denaturant-free buffer solution and removing precipitated impurities and growth hormone aggregates. The renatured growth hormone is then purified by ion-exchange chromatography.</p> |
申请公布号 |
EP0248618(B1) |
申请公布日期 |
1993.03.10 |
申请号 |
EP19870304818 |
申请日期 |
1987.06.01 |
申请人 |
PITMAN-MOORE INC. |
发明人 |
YANG,REN-DER;TABER,LARRY D;HAMILTON,EDWIN J.,JR. |
分类号 |
C12P21/00;C07K1/00;C07K1/113;C07K1/14;C07K1/18;C07K14/00;C07K14/575;C07K14/61;C07K19/00 |
主分类号 |
C12P21/00 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|