Human IgG1 monoclonal antibody specific for the nicotinic acetylcholine receptor and hybridoma producing the antibody

摘要

A human IgG1 type monoclonal antibody which possesses a molecular weight in the range of 180,000+/-20,000 as measured by the method of polyacrylamide gel electrophoresis performed in the presence of sodium dodecyl sulfate and is specific to nicotinic acetylcholine receptor. The human IgG1 type monoclonal antibody mentioned above is produced by a method which comprises fusing human cells capable of producing an antibody against nicotinic acetylcholine receptor with propagable human cells thereby giving rise to a hybridoma capable of producing the aforementioned human IgG1 type monoclonal antibody, selecting the hybridoma from the production of the fusion, culturing the hybridoma thereby giving rise to the aforementioned human IgG1 type monoclonal antibody, and selecting the antibody from the cultured medium. The hybridoma mentioned above is also identified.