| 摘要 |
The present invention is directed to a process for the analytical determination of acetylcyanamide in urine. It involves (a) the separating of acetylcyanamide at from a urine matrix and/or an extract which is obtained from a urine matrix by ion exchange chromatography or reverse phase high performance liquid chromatography (HPLC), and (b) detecting the acetylcyanamide at a wavelength of 220 nm. The matrix or the extract of it is cleaned by treatment with activated carbon at pH 7 to 13, and the acetylcyanamide may be extracted from the cleaned matrix or its extract at pH 0.5 to 3.5, e.g. by liquid-liquid extraction of the acetylcyanamide. This is carried out by means of water-insoluble, organic solvent such as ethyl acetate or diethyl ether.
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