| 摘要 |
DNA probes to detect Campylobacter jejuni and Campylobacter coli were prepared from a bacteriophage .lambda.gtll library by screening using an antiserum directed against a C. jejuni 46 kilodalton major outer membrane protein (MOMP). Two DNA fragments (MOMP DNA probes) were identified, cloned into pUC13 and characterized by restriction endonuclease digestion (Figure 1). One MOMP DNA probe (pDT1720) hybridized only to C. jejuni strains, whereas the other (pDT1719) hybridized to both C. jejuni and C. coli strains. The invention DNA probes can be used to specifically identify the aforementioned bacterial species when the species designation is in doubt and can also be used to identify the microorganisms directly in stool specimens, as disclosed herein. 28 Claims, 1 Drawing
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