NON-A NON-B HEPATITIS-RELATING NUCLEIC ACID, ANTIGEN, ANTIBODY AND THEIR DETECTION SYSTEM

摘要

PURPOSE:To provide a new DNA necessary for the diagnosis of non-A non-B hepatitis. CONSTITUTION:The GORgab DNA having the base sequence of formula. Nucleic acid is extracted from the plasma of chimpanzee, a cDNA is synthesized with a cDNA synthesis kit according to Gubler method, a cDNA library is prepared by using lambda gtll cloning kit and the cDNA is screened by a double antibody method (the 1st antibody is a mixture of plasma of chimpanzee infected with non-A non-B hepatitis, human plasma and plasma of patient of non-A non-B chronic hepatitis; the 2nd antibody is anti-human IgG labeled with a peroxidase). An insert DNA is incised from the obtained GOR 47-1 phage DNA. GOR 47-1 RNA and GOR 47-1 RNAC are prepared from the phage script recombinant using T3 and T2 promoter. The products are labeled with a radioactive isotope to obtain a probe, which is used in the preparation of plural cDNA clones having overlaps with GOR 47-1 from the cDNA library. The clones are linked with each other to obtain the objective GORgab DNA.