CELL CULTURE TANK.

摘要

A cell culture tank (A) having in the cell culture zone (A') thereof a partial zone (B) partitioned with a filter (8) permitting permeation of a culture medium and protein produced by cells under culture but intercepting permeation of cell or cohesive carrier of cells. A jet nozzle (6) of a conduit for feeding oxygen or air is provided on the bottom of the zone (B), stirring vanes (3) rotating at high speed are provided immediately above said jet nozzle, and a space corresponding to 30 to 50 % of the height of the filter (8) is exposed to a gaseous phase part in the cell culture tank. A conduit nozzle (7) for recovering the culture medium is provided just below the medium level in the zone (B). Another stirring vane (1) rotating at low speed is provided in the culture zone (A'). By providing an exhaust pipe (20) and a sterile air ventilation pipe (21) to the gaseous phase part of the culture zone (A') as well as valves to the conduit pipe of jet nozzle (6), exhaust pipe (20), and sterile ventilation pipe (21), respectively, the level of the medium in the zone (B) having been lowered by bubbles developing in the zone (B) is raised and said bubbles are squeezed out to the culture tank (A) through the filter, whereby the bubbles are broken. When an opening is provided in the upper part of the zone (B) and a bubble breaking vane rotating at high speed is provided above the level of medium in said zone (B), bubbles having developed in said zone can be broken.