| 摘要 |
<p>The process entails the fermentation mixture being circulated, at least for a time, and metabolic products of the cultivated cells and, where appropriate, biomass being separated, and has the following steps - charging of the fermentation system with an amount of nutrient medium which suffices to start the required cell culture; - sterilisation of the system and adjustment of the required nutrient medium concentration; - inoculation of the nutrient medium with the seed culture and leaving the culture to grow undisturbed for a defined period; - increasing the concentration of the nutrient medium to the specific nutrient medium concentration of the cell culture with, at the same time, increasing the nutrient medium volume to the working volume of the system and of the cell concentration; - changing to a continuous procedure with replacement of the nutrient medium and removal of the metabolic products, and complete or partial recycling of cells; - discontinuation of the continuous procedure at a required time and harvesting of the biomass under sterile conditions; and, where appropriate, - repetition of some or all of the abovementioned steps in the stated sequence, and apparatus for carrying out this process. <IMAGE></p> |
