| 摘要 |
PURPOSE:To efficiently obtain an amino acid composition containing a branched amino acid, such as (iso)leucine or valine, in high concentration and having an antioxidant action by hydrolyzing a vegetable protein and subjecting the hydrolyzate to column chromatographic treatment. CONSTITUTION:A vegetable protein, such as soybean, wheat or barley, is enzymatically or chemically hydrolyzed and the hydrolyzate is freed from solid matter before being subjected to column chromatographic treatment under acidic conditions. This technique makes it possible to recover branched amino acids, such as leucine, isoleucine an valine, contained in the vegetable protein each with a recovery of 40-100% and can give an amino acid composition containing 30-70wt.% branched amino acids, having a Fischer's ratio, which shows a weight ratio of branched amino acid to aromatic amino acid, of 3 to 22, and having an antioxidant action. The obtained composition is suitably used as an amino acid strengthening agent for, e.g. remedy for hepatic encephelopathy and application for imparting an antioxidant action to foods. |
