THIN LAYER CHROMATOGRAPHY DIRECT SAMPLE APPLICATION MANIFOLD

摘要

An apparatus and method for directly applying a liquid sample matrix containing analytes and possible endogenous compounds to selected areas of a thin layer chromatographic sheet. The apparatus comprises a manifold made from an upper and lower filter block which are fastened together and supported on a support member. The thin layer chromatographic sheet is held between the upper and lower filter block. Samples are applied to the thin layer chromatographic sheet through aligned through-bores in the upper and lower filter block by connecting a syringe to the through-bores of the upper filter block. The method involves initially wetting areas of the thin layer chromatographic sheet where the sample is subsequently applied. Analytes retained on the thin layer chromatographic sheet are subsequently analyzed and may be further extracted from the thin layer chromatographic sheet for analysis.