| 摘要 |
There is disclosed a method for isolating, renaturing and purifying recombinant interleukin-7 (IL-7) from prokaryotic transformed host cells that produce IL-7. The method for isolating purified recombinant IL-7 comprises entrapping the host cells in a porous matrix, wherein the porous matrix comprises a complex polysaccharide, extracting IL-7 from the porous matrix with an extraction buffer comprising a chaotropic agent and a reducing agent, renaturing extracted IL-7 by diluting IL-7 in a refolding buffer, and purifying renatured IL-7 from the refolding buffer. |
