发明名称 |
PROCESS FOR PRODUCING TISSUE PLASMINOGEN ACTIVATOR |
摘要 |
<p>This invention discloses a process for producing tissue plasminogen activator. The process comprises cultivating Escherichia coli, yeast, or mammalian cells into which the vector having the gene for human tissue plasminogen activator (tPA) has been introduced by the recombinant DNA technology. In the cultivation or purification process, a specific antiplasmin agent, which is one of the protease inhibitors, is added to the medium and/or the buffer solutions used for purification, so as to prevent the conversion of single-chain form tPA into double-chain form tPA by a protease which is present in the medium.</p> |
申请公布号 |
EP0208486(B1) |
申请公布日期 |
1993.02.10 |
申请号 |
EP19860305020 |
申请日期 |
1986.06.27 |
申请人 |
TOYO BOSEKI KABUSHIKI KAISHA;DAIICHI SEIYAKU CO., LTD. |
发明人 |
KOTANI, RYOTARO;UNUMA, TSUNEO;OTAWARA, SHIGEKI;KOBAYASHI, SESSUO;SUZUKI, TADAO |
分类号 |
A61K38/46;A61K35/12;C12N1/38;C12N9/64;C12N9/72;C12R1/91 |
主分类号 |
A61K38/46 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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