| 摘要 |
PURPOSE:To directly and effectively produce a brown alga decomposition product, oligosaccharides, originated from a brown alga without extracting polysaccharides, by decomposing the brown alga with a brown alga-decomposing enzyme produced by a microorganism belonging to the genus Alteromonas. CONSTITUTION:A lyophilized stored Alteromonas.SP 4778 strain as a microorganism belonging to the genus Alteromonas is inoculated on a medium and subsequently subjected to a shaking culture at 25 deg.C for 24hrs. The culture solution is centrifuged, and the supernatant is collected as a brown alga-decomposing enzyme solution. The enzyme solution, a brown alga such as MITSUISHI tangle and a 0.05M sodium phosphate buffer solution (pH7.5) are charged in a Eppendorf tube, reacted at 30 deg.C for 30min, heated in a boiling water bath for 10hr for stopping the enzymatic reaction, and subsequently centrifuged. The supernatant is separated, concentrated and purified by high performance liquid chromatography to provide the objective brown alga decomposition product comprising oligosaccharides, etc. |
