| 摘要 |
PURPOSE:To obtain simply, efficiently purified polypeptide of high activity in a short time by adding a modifier to a solution prepared by breaking polypeptide with an aqueous formic acid solution followed by treatment by a specific means to effect rapid removal of the formic acid. CONSTITUTION:Firstly, a modifier (pref. of low molecular weight, e.g. urea) is added to a solution prepared by breaking polypeptide with an aqueous formic acid solution (pref. 70% solution) either before, during or after the breaking. Thence, the system is put to gel filtration chromatography and eluted with a solution containing said modifier, thus separating the polypeptide. The gel to be used in the chromatography is pref. stable to acids. The concentration of the modifier in the elute is pref. the same as that of the sample. To eliminate the modifier from the fraction containing the objective polypeptide, dialysis is made in the modifier-free solution. |
