| 摘要 |
PURPOSE:To produce L-lysine in improved productivity and accumulation concen tration in a high yield by inoculating a L-lysine-producing strain on a liquid medium, culturing the strain, feeding a feed medium at a specific time so as to maintain the concentration of carbon at a specified value, and simultaneously culturing the strain. CONSTITUTION:A lysine-producing microorganism (e.g. Brevibacterium.lactofermentum ATCC 21800) is inoculated on a perfect liquid medium, cultured and subjected to a logarithmic growth. The culture solution is subsequently mixed with a feed medium containing both a carbon source (e.g. sugar or organic acid) and a nutrient having a growth-accelerating effect (e.g. soy bean protein hydrolysate or yeast extract) and is fed to culture so that the concentration of the carbon source in the culture solution is maintained at <=5g/l, followed by collecting the objective L-lysine. |
