| 摘要 |
<p>The invention provides a method to determine the amount of a target nucleic acid in a sample which comprises (i) mixing the sample with a predetermined amount of a control nucleic acid, the control nucleic acid having a control region which includes at least one primer binding region homologous to a primer binding region in a selected region of the target nucleic acid; (ii) bringing the mixture formed in (i) into contact with at least one nucleic acid primer capable of binding to the primer binding region of the target and control nucleic acids; (iii) performing a nucleic acid amplification reaction, said reaction requiring the presence of the primer to amplify the selected region of the target nucleic acid and the control region of the control nucleic acid; (iv) determining the relative quantities of the amplified control region and selected region nucleic acids; and (v) calculating from the determination of (iv) the amount of target nucleic acid in the sample. The method can be used for the quantitation of a PCR, e.g. using a control nucleic acid which has a centrally located unique restriction site not present in the target nucleic acid. Target nucleic acids may include viral genomes of e.g. CMV.</p> |
