| 摘要 |
<p>A method for assessing the production by living cells of one or more molecules such as soluble mediators or infectious particle elements, wherein cell culture and optionally stimulation are performed to induce said production and the number of molecules thereby produced is assessed by an in vitro solid phase immunoassay, and wherein a) the cell culture and optionally the stimulation are performed, optionally in the presence of a tracer, in a container which includes the solid phase of the assay on which the capture elements specific for the molecule(s) to be assayed are fixed, and b) when the culturing time is over, and after the solid phase has been washed and any other reagents required for the assay, particularly a tracer substrate, have been incubated in the presence of the tracer, the signal emitted by the tracer fixed to the solid phase, or by said substrate thereof, is read. A technological format which is useful in said method is also provided.</p> |
