COMPOSITIONS AND METHODS FOR REPRODUCING POSITIVE DIAGNOSTIC INDICATIONS

摘要

The invention provides a functional vector for reproducing positive diagnostic indications of one or more infectious or pathogenic organisms. The vector includes a viral genome having a deletion of a native sequence and an insertion of one or more heterologous sequences characteristic of the infectious or pathogenic organism. Host cells transformed with the functional vector and recombinant viruses produced by such transformed host cells are also provided. A method for detecting one or more target sequences indicative of one or more infectious or pathogenic organisms is provided. The method includes (a) providing a self-replicating functional vector. The vector includes a viral genome having a deletion of a native sequence and an insertion of one or more heterologous sequences characteristic of the infectious or pathogenic organism; (b) introducing the self-replicating functional vector into compatible host cells to produce infected cells capable of generating recombinant viruses having the self-replicating functional vector as their viral genome; (c) fixing the infected cells capable of generating recombinant viruses; and (d) detecting the one or more heterologous sequences characteristic of the infectious or pathogenic organism in the fixed infected cells.