RECOMBINANT SECRETABLE FUSION PROTEINS

摘要

A method for producing a single chain fusion protein, comprises the steps of: (a) constructing an rDNA molecule coding for a secretable single chain fusion protein having a plurality of functional proteins or protein domains joined by spacer peptides, wherein each such spacer peptide comprises a flexible hinge region of a naturally secreted multidomain protein or an analogue thereof, each adjacent pair of functional proteins or protein domains is joined by a spacer peptide which is not naturally continuous with either functional protein or protein domain to which it is joined, and at least two of the functional proteins or protein domains each independently exhibit physicochemical or biological activity or interact to form an assembly that exhibits physicochemical or biological activity; (b) inserting the rDNA molecule into an expression vector capable of transforming a host organism; (c) transforming a host organism with the resultant vector, and isolating transformants; and (d) culturing the transformants and recovering thesingle chain fusion protein.