摘要 |
Novel compounds and methods for the formation of disulfide linkages are presented. The novel compounds employed are substituted 2-iminothiolane hydrohalide linking agents of the following formula (I): <IMAGE> (I) wherein, X is halogen; R1 is COOR5; halogen; nitro; unsubstituted or halogenated C1-8alkyl; unsubstituted or halogenated C1-8alkoxy; unsubstituted or halogenated C2-8alkenyl; unsubstituted or halogenated C2-8alkynyl; unsubstituted C3-8cycloalkyl; unsubstituted aryl; aryl substituted with 1 to 3 substituents selected from halogen, amino, unsubstituted or halogenated C1-8alkyl, or unsubstituted or halogenated C1-8alkoxy; unsubstituted heterocycle; or heterocycle substituted with 1 to 3 substituents selected from amino, halogen, unsubstituted or halogenated C1-8alkyl, or unsubstituted or halogenated C1-8alkoxy; each of R2, R3 and R4 is independently hydrogen or selected from the values of R1; or R1 and R2 together form a C2-5alkylene bridge, unsubstituted or substituted with one to five C1-4alkyl groups; or R1 or R2 together with R3 form a C1-5alkylene bridge, unsubstituted or substituted with one to five C1-4alkyl groups; and R5 is hydrogen or C1-8alkyl. Also included in the invention are methods for covalently linking two species, which methods comprise reacting one or both of the two species with a crosslinking agent of the above formula (I) and mixing the two species together. The resulting conjugated species containing the linking group are also included in the invention. Further included in the invention is the use of a quenching agent which allows direct measurement of the reaction of 2-iminothiolanes with nucleophilic groups.
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