A kit and a method for detecting denatured lipoproteins is disclosed. The method comprises: adding to a sample a mixture of (i) an affinity gel with which a monoclonal antibody for apoprotein A-I is coupled and (ii) an affinity gel with which a monoclonal antibody for apoprotein B-100 is coupled; removing apoprotein A-I and apoprotein B-100 in the sample by combining the apoprotein A-I and apoprotein B-100 with the affinity gels; and measuring an amount of lipoproteins in the residue. The kit comprises a mixture of (i) and (ii) as an essential component. The kit and the method is useful for the clinical investigation and diagnosis of abnormal lipid metabolism which can be a cause of heart diseases (e.g. cardiac infarction), diabetes, and various types of atherosclerosis diseases.
申请公布号
US5183738(A)
申请公布日期
1993.02.02
申请号
US19890413484
申请日期
1989.09.27
申请人
JAPAN IMMUNO RESEARCH LABORATORIES CO., LTD.
发明人
ADACHI, MASAKAZU;SAITOU, TOSHIMITU;MURATA, KIMIYO;TAMURA, AKI