发明名称 |
DICTYOSTELID EXPRESSION VECTOR AND METHOD FOR EXPRESSING A DESIRED PROTEIN |
摘要 |
The invention relates to recombinant DNA molecules comprising a Dictyostelium discoideum homologous promoter region, a heterologous DNA sequence with a Dictyostelium discoideum homologous peptide sequence capable of functioning as a leader peptide sequence positioned upstream thereof and in proper reading frame therewith, and a Dictyostelium discoideum homologous termination region, the heterologous DNA sequence encoding the desired functional polypeptide or intermediate thereof, said DNA sequence being positioned downstream from the promoter region and said termination region being positioned downstream from the DNA sequence. The invention also provides recombinant dictyostelid hosts as well as methods for preparing recombinant DNA molecules of the invention and for expressing recombinant polypeptides encoded by said recombinant DNA molecules in dictyostelid hosts. The invention in particular relates to the expression of the CS protein of Plasmodium falciparum. |
申请公布号 |
WO9220806(A3) |
申请公布日期 |
1993.01.21 |
申请号 |
WO1992EP01112 |
申请日期 |
1992.05.18 |
申请人 |
RMF DICTAGENE S.A. |
发明人 |
FASEL, NICOLAS, JOSEPH;REYMOND, CHRISTOPHE, DOMINIQUE |
分类号 |
C12N1/15;C07K14/445;C12N9/10;C12N15/09;C12N15/30;C12N15/80;C12P21/00;C12P21/08;C12R1/645 |
主分类号 |
C12N1/15 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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