ASSAY METHODS AND COMPOSITIONS FOR DETECTING SERUM PROTEASES, PARTICULARLY ACTIVATED PROTEIN C

摘要

2112702 9301309 PCTABS00019 The invention describes diagnostic methods and compositions for determining the amount of protease in a body fluid sample. In particular, the invention detects proteases by a method in which both a reversible inhibitor of the protease and an irreversible inhibitor of interfering protease during the detection step are employed to increase the sensitivity of the enzyme capture assay. The assay detects normal serum levels of activated protein C.