EXPRESSION VECTORS CONTAINING THE HEAT SHOCK REGULATORY DNA SEQUENCES FROM A HEAT SHOCK PROTEIN 83 (HSP83) GENE AND INDUCIBLE EXPRESSION BY THE USE OF THE VECTORS

摘要

Expression vectors are constructed in which a desired foreign structural gene which encode, for example, physiological active substance such as human tissue plasminogen activator is functionally linked to heat shock regulatory DNA sequences from Drosophila melanogaster approximately 83 k daltons heat shock protein (hsp83) gene or the modified DNA sequences of the regulatory DNA sequences and other DNA sequences necessary for translation of the desired foreign structural gene. Cells transformed with the expression vector are grown and the production of the desired structural gene products by the transformed cells is induced by heat shock treatment when necessary.