| 摘要 |
Disclosed is a vaccine for the prevention of disease caused by canine distemper virus (CDV). The vaccine is prepared from CDV immunogens derived from CDV persistently-infected cells cultured in vitro. CCL-64 mink lung cells are the preferred cell line. CDV persistently-infected cells are cultured in serum-containing growth medium, the cells transferred and maintained in serum-free medium under conditions and for a time adequate to accumulate in said serum-free medium said CDV immunogens, and the cells separated from said CDV immunogen-containing supernatant. The CDV vaccine is made by diluting said supernatent and blending with a pharmaceutically-acceptable adjuvant.
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