发明名称 |
IMPROVEMENT OF THE YIELD WHEN DISULFIDE-BONDED PROTEINS ARE SECRETED |
摘要 |
The invention relates to a method for enhancing the development of the natural protein conformation on secretion of proteins with a disulphide bridge by a prokaryotic host organism which contains a recombinant DNA which codes for the secreted protein, in which the host organism is cultured in a suitable nutrient medium under suitable conditions for the expression of the recombinant DNA, which is characterised in that a nutrient medium containing 0.1 to 20 mmol/l of one or more thiol reagents is used. |
申请公布号 |
ZA9202971(B) |
申请公布日期 |
1992.12.30 |
申请号 |
ZA19920002971 |
申请日期 |
1992.04.24 |
申请人 |
BOEHRINGER MANNHEIM GMBH. |
发明人 |
RUDOLF GLOCKSHUBER;ARNE SKERRA;MARTINA WUNDERLICH;RAINER RUDOLPH |
分类号 |
C12N15/09;C07K1/113;C07K14/81;C12N1/38;C12N9/90;C12N15/15;C12N15/61;C12P1/00;C12P21/02;C12P21/04;C12R1/19 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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