CONFOCAL SPECTROSCOPY

摘要

A spot (19) of a sample (18) is illuminated by laser light (10). Raman scattered light is collimated in a parallel beam by a microscope objective (16), and analysed by a dispersive or non-dispersive analyser (20) (such as a diffraction grating or filter). A lens (22) then focuses the Raman scattered light onto a two-dimensional photodetector array in the form of a charge-coupled device (CCD) (24). A confocal technique is described to eliminate light (36) scattered from outside the focal plane of the objective (16). This may be done by binning together a few pixels of the CCD at the focal point (26) of the lens (22), or by image processing techniques in a computer (25).