MAJOR SECRETION PRODUCT (MSP) OF LACTOCOCCUS LACTIS, DNA MOLECULES CONTAINING MULTIFUNCTIONAL ORIGINS OF REPLICATION AND/OR ENCODING MSP, VECTORS AND HOST CELLS

摘要

The invention relates to the field of genetic engineering and provides novel DNA molecules which comprise multifunctional origins of replication and/or a DNA sequence coding for the most abundant protein in the supernatant of cultures of Lactococcus spec., named Major Secretion Product (MSP), for its signal peptide and/or the promoter of the MSP gene. The novel DNA molecules are used for the production of novel shuttle vectors for cloning of DNA in at least E. coli and Lactococcus spec. or novel vectors for the expression of homologous or heterologous genes and the production of secreted gene products in gram positive bacteria such as Lactococcus spec. and Bacillus spec. <IMAGE>