发明名称 |
Assay method for hydrolytic enzymes. |
摘要 |
<p>An assay method for the rapid determination of hydrolytic enzyme activity in large numbers of samples is provided which comprises bonding a resin-binding compound, such as biotin, to one side of the scissile bond of the substrate and a reporter molecule, such as a fluorescence marker, to the opposite side of the scissile bond, incubating the modified substrate and the enzyme in multiple well plates, e.g. 96-well plates, optionally in the presence if a test inhibitor or activator compound transferring the incubation solutions to a second multiple well plate having upper and lower chambers separated by a porous membrane the upper chamber of which contains resin beads capable of binding with the resin-binding compound, filtering and washing the wells of the second plate and reading the emission from the plates. The invention also provides protease substrates for HIV-1 protease, vertebrate stromelysin and derivatives thereof which are useful in the assay method.</p> |
申请公布号 |
EP0518557(A2) |
申请公布日期 |
1992.12.16 |
申请号 |
EP19920305109 |
申请日期 |
1992.06.03 |
申请人 |
ELI LILLY AND COMPANY |
发明人 |
HEATH, WILLIAM FRANCIS, JR.;LAI, MEI-HUEI TSAI;MANETTA, JOSEPH VINCENT;SPORTSMAN, JOHN RICHARD;YAN, SAU-CHI BETTY |
分类号 |
C07K7/08;C07K7/06;C07K7/22;C07K14/16;C07K14/78;C12Q1/34;C12Q1/37;G01N21/78;G01N33/573 |
主分类号 |
C07K7/08 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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